36OR
Room-temperature multiconformer model of HRas-GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2025-02-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97935 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 89.967, 89.967, 136.406 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.460 - 1.900 |
| R-factor | 0.1374 |
| Rwork | 0.136 |
| R-free | 0.17330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.460 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.292 | 3.366 |
| Rmeas | 0.308 | 3.543 |
| Rpim | 0.095 | 1.102 |
| Total number of observations | 219881 | 31656 |
| Number of reflections | 21304 | 3090 |
| <I/σ(I)> | 5.7 | 0.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 10.3 | 10.2 |
| CC(1/2) | 0.995 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 3350, 0.2M Calcium chloride, pH 8.0 |
