36LY
Crystal Structure of mini-binder MB07
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.91992 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.042, 34.711, 37.038 |
| Unit cell angles | 81.61, 70.99, 72.00 |
Refinement procedure
| Resolution | 32.970 - 2.550 |
| R-factor | 0.2288 |
| Rwork | 0.223 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.515 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.970 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.088 | 0.458 |
| Rpim | 0.085 | 0.498 |
| Number of reflections | 4566 | 581 |
| <I/σ(I)> | 4.1 | 1.5 |
| Completeness [%] | 94.7 | 98.5 |
| Redundancy | 2.7 | 2.9 |
| CC(1/2) | 0.965 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.2 M Lithium sulfate, 0.1 M Sodium acetate pH 4.5, and 50 % w/v PEG 400 |
