36GK
D-GlcNAc-bound structure of Vibrio vulnificus putative carbohydrate binding module and split domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-03-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95371 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 120.580, 189.490, 199.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.810 - 2.280 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.22267 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.381 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 48.800 | 2.340 |
| High resolution limit [Å] | 2.280 | 6.000 | 2.280 |
| Rmerge | 0.115 | 0.061 | 3.216 |
| Rmeas | 0.120 | 0.064 | 3.348 |
| Number of reflections | 103968 | 5967 | 7711 |
| <I/σ(I)> | 12.94 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 13.7 | ||
| CC(1/2) | 0.998 | 0.998 | 0.353 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295.15 | 0.2 M Sodium acetate, 0.1 M Bis-tris propane pH 7.5, 20% w/v PEG 3350 |
