36AD

Fumarate hydratase (human mitochondrial)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-11-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978720
Spacegroup name	P 32 2 1
Unit cell lengths	189.490, 189.490, 115.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.980 - 2.110
R-factor	0.1809
Rwork	0.179
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.508
Data reduction software	xia2 (3.21.1)
Data scaling software	xia2 (3.21.1)
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.370	2.150
High resolution limit [Å]	2.110	2.110
Rmerge	0.209	3.634
Number of reflections	137305	6746
<I/σ(I)>	12.3	0.6
Completeness [%]	100.0
Redundancy	21.2
CC(1/2)	0.999	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.2 M sodium potassium tartrate 01. M Bis-Tris pH 6.5 10% PEG 10,000