Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 21.162, 28.670, 44.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.300 |
| R-factor | 0.12 * |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODIFIED NDB ENTRY ZDF002 (PDB ENTRY 1DCG) |
| RMSD bond length | 0.000 |
| RMSD bond angle | 0.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-96 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.043 | 0.078 |
| Number of reflections | 7024 | |
| <I/σ(I)> | 20 | 23.6 |
| Completeness [%] | 99.2 | 87.4 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | 291 * | drops of 6 micro litter total volume obtained by combining 3 micro litter of stock solution with 3 micro litter of mother liquor * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | single-stranded hexamer | 2 (mM) | |
| 2 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 3 | 1 | reservoir | cobalt hexammine | 1 (mM) | |
| 4 | 1 | reservoir | argininamide chloride | 5 (mM) | |
| 5 | 1 | reservoir | MPD | 30 (%(v/v)) | |
| 6 | 1 | reservoir | PEG400 | 5 (%(v/v)) |
