35UG
Human DVL2 PDZ domain in complex with Vangl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-27 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.330, 52.320, 77.520 |
| Unit cell angles | 90.00, 104.21, 90.00 |
Refinement procedure
| Resolution | 33.610 - 1.950 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.612 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.320 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.055 | 0.231 |
| Rpim | 0.023 | 0.132 |
| Number of reflections | 33128 | 4782 |
| <I/σ(I)> | 21.1 | 3.1 |
| Completeness [%] | 98.1 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES pH 7.5, 24% w/v Polyethylene glycol 10000 |
