35SL

Polar Interface Homodimer - S2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-03-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	43.578, 43.578, 151.542
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.740 - 1.800
R-factor	0.1894
Rwork	0.184
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.302
Data reduction software	PROTEUM PLUS (2025.6-0)
Data scaling software	PROTEUM PLUS (2025.6-0)
Phasing software	PHASER
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.740	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	29399	1260
<I/σ(I)>	14.13
Completeness [%]	98.5
Redundancy	4.9
CC(1/2)	0.998	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	10% w/v 2-Propanol, 0.1 M Bicine, 30% w/v Polyethylene Glycol 1,500