35C8
CATALYTIC ANTIBODY 5C8, FAB-INHIBITOR COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 97 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.200, 79.900, 65.000 |
| Unit cell angles | 90.00, 118.20, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.198 * |
| Rwork | 0.206 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FREE FAB 5C8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 30.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 * | 0.387 * |
| Total number of observations | 249541 * | |
| Number of reflections | 33264 | |
| <I/σ(I)> | 20 | 3.2 |
| Completeness [%] | 98.0 | 97.6 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.5 | 22.5 * | pH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG10000 | 14 (%) | |
| 4 | 1 | reservoir | Tris-malate | 0.2 (M) |
