359D
INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.540, 65.540, 138.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.800 - 2.900 |
| R-factor | 0.256 |
| Rwork | 0.256 |
| R-free | 0.30200 |
| Starting model (for MR) | NDB ENTRY URX057 (PDB ENTRY 299D) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 14.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR (REFINEMENT BASED ON INITIAL MODEL PHASES) |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.800 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.052 | 0.082 |
| Total number of observations | 30459 * | |
| Number of reflections | 7810 | |
| <I/σ(I)> | 7.7 | 4.8 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 3.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | Scott, W.G., (1996) Science, 274, 2065. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | LI SULFATE | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | TBCL3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 2 | 1 | EDTA | 1.25 (mM) | ||
| 3 | 1 | sodium cacodylate | 50 (mM) |
