358D
CRYSTAL STRUCTURE OF THE 2:1 NETROPSIN-DNA DECAMER D(CBRCCCCIIIII) COMPLEX WITH END-TO-END BINDING
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 61 |
| Unit cell lengths | 32.130, 32.130, 143.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.29800 |
| Starting model (for MR) | D(CCCCCIIIII) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.690 |
| Data reduction software | MSC |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 2.670 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.059 | 0.139 |
| Number of reflections | 1746 | |
| <I/σ(I)> | 3.14 | 3.1 |
| Completeness [%] | 60.3 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 291 * | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 0.3 (mM) | |
| 2 | 1 | drop | netropsin | 0.3 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 0.4 (mM) | |
| 4 | 1 | drop | cobaltic hexamine chloride | 0.06 (mM) | |
| 5 | 1 | reservoir | MPD | 60 (%) |
