34YP
Substrate-binding domain of S. cerevisiae Ssc1 in complex with peptide p5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-19 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.033220 |
| Spacegroup name | P 43 |
| Unit cell lengths | 72.040, 72.040, 93.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.780 - 2.400 |
| R-factor | 0.2213 |
| Rwork | 0.218 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.416 |
| Data reduction software | XDS (20220820) |
| Data scaling software | XDS (20220820) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.780 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.068 | 0.849 |
| Rmeas | 0.071 | 0.880 |
| Number of reflections | 18801 | 1883 |
| <I/σ(I)> | 28.77 | 3.34 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 14.3 | 14.4 |
| CC(1/2) | 1.000 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291.15 | 0.1 M sodium cacodylate, 0.2 M magnesium acetate, 25 % (w/v) PEG 8000 |
