34FS
Pseudo-merohedral twinning crystal form of putative amidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-16 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 111.430, 193.250, 436.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.630 - 1.700 |
| R-factor | 0.2247 |
| Rwork | 0.222 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.293 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.630 | 1.700 |
| High resolution limit [Å] | 1.697 | 1.697 |
| Number of reflections | 509143 | 78140 |
| <I/σ(I)> | 21.92 | |
| Completeness [%] | 99.1 | |
| Redundancy | 13.7 | |
| CC(1/2) | 1.000 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v PEG 3,350; 0.2 Magnesium Acetate |
