34CV

Homo sapiens Eukaryotic translation initiation factor 4E (eIF4E) in complex with synthetic mRNA cap analogs

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	93
Detector technology	PIXEL
Collection date	2025-10-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976
Spacegroup name	P 1
Unit cell lengths	38.060, 38.109, 146.620
Unit cell angles	84.37, 88.48, 77.01

Refinement procedure

Resolution	36.990 - 1.750
R-factor	0.2489
Rwork	0.247
R-free	0.28369
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.601
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0431)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.020	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.072	1.008
Rmeas	0.078	1.099
Rpim	0.029	0.430
Total number of observations	492925	18536
Number of reflections	73204	3092
<I/σ(I)>	13.4	1.6
Completeness [%]	92.1
Redundancy	6.7	6
CC(1/2)	0.998	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	281	18 % w/v PEG 10,000; 20 % w/v Glycerol; 100 mM TRIS; pH 8.5; 100 mM Sodium chloride