348D
X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: LOW SALT CONCENTRATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-01-05 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 38.606, 38.606, 80.573 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 1.700 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.21000 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.640 |
| Data reduction software | XENGEN |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 * |
| Rmerge | 0.052 |
| Number of reflections | 4447 |
| Completeness [%] | 97.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 10 (mM) | |
| 2 | 1 | drop | cacodylate acid | 200 (mM) | |
| 3 | 1 | drop | MPD | 30 (%(v/v)) | |
| 4 | 1 | drop | 7.8 (mM) | ||
| 5 | 1 | drop | spermine | 7.8 (mM) | |
| 6 | 1 | reservoir | cacodylate acid | 200 (mM) | |
| 7 | 1 | reservoir | MPD | 30 (%(v/v)) |
