33VD

SAD-phased structure of SARS-CoV-2 Nucleocapsid protein CTD.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I23
Synchrotron site	Diamond
Beamline	I23
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-05-06
Detector	DECTRIS PILATUS 12M
Wavelength(s)	1.9074
Spacegroup name	P 43
Unit cell lengths	88.500, 88.500, 39.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.250 - 1.230
Rwork	0.148
R-free	0.17410
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.977
Data reduction software	XDS (Jan 19, 2025)
Data scaling software	Aimless (0.8.2)
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.142))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.250	1.250
High resolution limit [Å]	1.230	1.230
Number of reflections	82406	2966
<I/σ(I)>	22.4
Completeness [%]	92.8
Redundancy	6.7
CC(1/2)	1.000	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	0.1 M HEPES, pH 7.8 10 % isopropanol 23% w/v PEG 4000