33QJ

Cytochrome P450 Reductases in complex with NADPH from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-12-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	104.568, 106.000, 116.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.610 - 2.150
R-factor	0.20594
Rwork	0.203
R-free	0.25249
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.339
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.610	2.190
High resolution limit [Å]	2.150	2.150
Rmeas	0.282
Number of reflections	70690	70690
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	13.7
CC(1/2)	1.000	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M calcium chloride dihydrate, 20 % w/v polyethylene glycol 3350