333D
THE CRYSTAL STRUCTURE OF AN RNA OLIGOMER INCORPORATING TANDEM ADENOSINE-INOSINE MISMATCHES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-04-21 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 39.050, 39.050, 58.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 2.520 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CGC:GCG FRAGMENT OF ARL048 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.400 |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 * |
| High resolution limit [Å] | 2.520 |
| Rmerge | 0.079 |
| Total number of observations | 5952 * |
| Number of reflections | 1014 |
| Completeness [%] | 91.8 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | pH 7.50, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 1 | HEPES | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | PEG 400 | ||
| 7 | 1 | 2 | CACL2 | ||
| 8 | 1 | 2 | HEPES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.5 (mM) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | reservoir | PEG400 | 28 (%) |
