332D
CRYSTAL STRUCTURE OF R(GUGUGUA)D(C) WITH TANDEM G-U/U-G WOBBLE PAIRS WITH STRAND SLIPPAGE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | H 3 |
| Unit cell lengths | 40.820, 40.820, 66.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.580 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AHH077 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XENGEN (V. 2.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.580 * |
| Rmerge | 0.047 * |
| Number of reflections | 4314 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | [CO(NH3)6]3+ | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 1 (mM) | single strand concentration |
| 2 | 1 | drop | cobaltic hexammine chloride | 0.5 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 4 | 1 | drop | MPD | 10 (%(v/v)) | |
| 5 | 1 | reservoir | MPD | 55 (%) |
