32TC

Crystal structure of the human SPRY domain-containing SOCS box protein SPSB4 bound to fragment Z104474228

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-01-26
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9156
Spacegroup name	C 2 2 21
Unit cell lengths	63.617, 84.000, 68.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.810 - 1.560
R-factor	0.2047
Rwork	0.202
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.999
Data reduction software	xia2
Data scaling software	xia2
Phasing software	DIMPLE
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.810	1.590
High resolution limit [Å]	1.560	1.560
Rpim	0.081	0.880
Number of reflections	26521	1285
<I/σ(I)>	4.6	0.4
Completeness [%]	100.0	99
Redundancy	12.4
CC(1/2)	0.993	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M Sodium Citrate pH 5.5, 20% PEG3000