32QU

Human NUP98 APD (SG P41212)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	60.499, 60.499, 89.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.651 - 1.500
Rwork	0.192
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.377
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.170	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.125	1.765
Rmeas	0.127	1.799
Rpim	0.025	0.346
Total number of observations	715561	35381
Number of reflections	27463	1324
<I/σ(I)>	16.2	2.1
Completeness [%]	100.0
Redundancy	26.1	26.7
CC(1/2)	0.999	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	25% PEG 3350 0.2 MgCl2 0.1 M HEPES pH 6.8