32PV

human CSTF50 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	35.759, 65.591, 160.512
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.720 - 1.560
R-factor	0.203
Rwork	0.202
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.153
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.260	1.650
High resolution limit [Å]	1.560	1.560
Rmeas	0.061	0.630
Rpim	0.026	0.273
Number of reflections	54800	50580
<I/σ(I)>	14.5	2.4
Completeness [%]	100.0	100
Redundancy	6.4	6.5
CC(1/2)	0.999	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	25% (w/v) PEG3350, 0.2 M NaCl, 0.1 M Na-HEPES pH 7.5