32OA
The ultra-high-resolution hyaluronan-binding domain of mouse CD44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-11 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.7338 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.960, 82.136, 32.225 |
| Unit cell angles | 90.00, 117.88, 90.00 |
Refinement procedure
| Resolution | 41.068 - 0.775 |
| Rwork | 0.110 |
| R-free | 0.11560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.756 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.068 | 0.826 |
| High resolution limit [Å] | 0.775 | 0.775 |
| Rmerge | 0.276 | 5.997 |
| Rmeas | 0.277 | 6.072 |
| Rpim | 0.029 | 0.932 |
| Number of reflections | 132017 | 2643 |
| <I/σ(I)> | 14.1 | 1.6 |
| Completeness [%] | 80.5 | 9.3 |
| Redundancy | 86.9 | 39.4 |
| CC(1/2) | 1.000 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 200 mM ammonium sulphate, 100 mM MES, 24% PEG5KMME |
