32NZ
An activating H90 ScFv bound to the hyaluronan-binding domain of human CD44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.538, 92.538, 109.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.210 - 1.800 |
| Rwork | 0.194 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.414 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.150 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.095 | 5.087 |
| Rmeas | 0.097 | 5.272 |
| Rpim | 0.020 | 1.354 |
| Number of reflections | 44688 | 2605 |
| <I/σ(I)> | 20.5 | 0.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 44.8 | 27.4 |
| CC(1/2) | 1.000 | 0.414 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM HEPES, 1% PEG 2000 MME, 1 M succinic acid |
