329D
EFFECT OF CYTOSINE METHYLATION ON DNA-DNA RECOGNITION AT CPG STEPS
Experimental procedure
| Temperature [K] | 273 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-03-01 |
| Detector | SIEMENS |
| Spacegroup name | H 3 |
| Unit cell lengths | 65.530, 65.530, 46.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BDL035 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.028 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.500 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.054 |
| Number of reflections | 1789 |
| Completeness [%] | 95.0 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 15 * | pH 6.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | MG ACETATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dodecamer duplex solution | 0.5 (mM) | |
| 2 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 3 | 1 | reservoir | magnesium acetate | 10 (mM) | |
| 4 | 1 | reservoir | MPD | 30 (%) |
