31JT

SFX structure of non-photoactivated PAmCherry1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL2
Synchrotron site	SACLA
Beamline	BL2
Temperature [K]	293.15
Detector technology	CCD
Collection date	2026-02-05
Detector	MPCCD
Wavelength(s)	1.24
Spacegroup name	P 2 21 21
Unit cell lengths	39.360, 79.160, 145.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.800 - 1.630
R-factor	0.1645
Rwork	0.163
R-free	0.19430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.997
Data reduction software	CrystFEL (0.12.0)
Data scaling software	CrystFEL (0.12.0)
Phasing software	PHENIX ((1.20.1_4487: 000))
Refinement software	PHENIX ((1.20.1_4487: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.700	1.670
High resolution limit [Å]	1.630	1.630
Number of reflections	57808	3823
<I/σ(I)>	8.108
Completeness [%]	100.0
Redundancy	1042.122
CC(1/2)	0.990	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293.15	0.2 M sodium acetate, 30% (w/v) PEG 3350, and 25 mg/ml protein solution