317D
STRUCTURE OF D(CCTAGGG): COMPARISON WITH NINE ISOMORPHOUS OCTAMER SEQUENCES REVEALS FOUR DISTINCT PATTERNS OF SEQUENCE-DEPENDENT INTERMOLECULAR INTERACTIONS
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-03-01 |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.220, 42.220, 24.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| Starting model (for MR) | ADH012 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 3.510 |
| Data reduction software | XENGEN (V. 2.0) |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.029 |
| Number of reflections | 1894 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 295 | pH 7.00, VAPOR DIFFUSION, temperature 295.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | DNA | 2 (mM) | single strand concentration |
| 4 | 1 | drop | spermine tetrachloride | 1 (mM) | |
| 5 | 1 | reservoir | MPD | 40 (%(v/v)) |
