312D
Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WATSON-CRICK BASE PAIR
Experimental procedure
| Source type | SEALED TUBE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-05-01 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 20.320, 29.540, 51.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ZDGB55 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.200 | 14.200 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.083 * | |
| Total number of observations | 20832 * | |
| Number of reflections | 5340 | |
| <I/σ(I)> | 9.8 | 3.37 |
| Completeness [%] | 84.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | [CO(NH3)6]3+ | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.5 (mM) | single stranded |
| 2 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | cobalt hexammine | 2.5 (mM) | |
| 5 | 1 | drop | MPD | 5 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 17 (%) |
