30KX
Complex of Colletotrichum higginsianum effector protein ChEC108 with HMA domain 1 (HMA1) of HIPP6 from Arabidopsis thaliana.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.914, 76.639, 99.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.740 - 2.750 |
| R-factor | 0.20926 |
| Rwork | 0.205 |
| R-free | 0.29487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.339 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.770 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.189 | 0.917 |
| Rmeas | 0.201 | 0.969 |
| Rpim | 0.066 | 0.311 |
| Total number of observations | 9622 | |
| Number of reflections | 7405 | 1060 |
| <I/σ(I)> | 7.9 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 8.7 | 9.1 |
| CC(1/2) | 0.993 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG MME2K with 0.1 M HEPES |
