30ID

Recombinant Streptomyces plicatus EndoH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-07
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	57.357, 87.396, 217.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	108.560 - 1.986
R-factor	0.2209
Rwork	0.219
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	autoPROC (1.1.7 (20210504))
Data scaling software	autoPROC (1.1.7 (20210504))
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	108.561	108.561	2.081
High resolution limit [Å]	1.986	5.841	1.986
Rmerge	0.169	0.053	1.165
Rmeas	0.200	0.062	1.365
Rpim	0.106	0.033	0.704
Number of reflections	64941	3246	3247
<I/σ(I)>	7.6	20.8	1.4
Completeness [%]	85.0	99.8	32.8
Redundancy	6.6	6.3	6.9
CC(1/2)	0.997	0.999	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2M Magnesium chloride, 0.1 M TRIS pH 8.5, 25% (w/v) PEG 3350 (Index H1)