30CP

Structure of the Hepatitis C Virus E2 Core from Genotype 6a in Complex with Germline Reverted Variants of the Broadly Neutralizing Antibody AR3C

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-29
Detector	DECTRIS PILATUS4 X 4M
Wavelength(s)	0.96770
Spacegroup name	P 21 21 21
Unit cell lengths	60.334, 64.450, 183.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.050 - 1.930
R-factor	0.204
Rwork	0.202
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.097
Data reduction software	EDNA
Phasing software	PHASER (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.050	2.000
High resolution limit [Å]	1.930	1.930
Rmerge	0.120
Number of reflections	54095	5240
<I/σ(I)>	7
Completeness [%]	94.8
Redundancy	4.4
CC(1/2)	0.990	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES (pH 7.5), 20% (w/v) PEG 4000, and 10% (v/v) isopropanol