307D
Structure of a DNA analog of the primer for HIV-1 RT second strand synthesis
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.820, 28.280, 72.490 |
| Unit cell angles | 90.00, 103.87, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.233 |
| Structure solution method | MIR PHASE ANALYSIS |
| Starting model (for MR) | IDEAL B-DNA HELIX OF SEQUENCE CAAAGAAAAG |
| RMSD bond length | 0.015 |
| RMSD bond angle | 15.950 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.058 * | |
| Number of reflections | 9686 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 93.8 | 91 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 * | VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMIDINE | ||
| 5 | 1 | 1 | BETA-OCTAGLUCOSIDE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA decamer | 0.17 (mM) | double helix |
| 2 | 1 | drop | magnesium acetate | 1.53 (mM) | |
| 3 | 1 | drop | spermidine | 0.17 (mM) | |
| 4 | 1 | drop | beta-octaglucoside | 0.05 (%(v/v)) | |
| 5 | 1 | drop | MPD | 9.5 (%(v/v)) |
