304D
SIDE-BY-SIDE BINDING OF DISTAMYCIN MOLECULES TO D(ICATATIC) IN THE MONOCLINIC FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1994-11-03 |
Detector | SIEMENS-NICOLET |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 33.380, 25.330, 28.110 |
Unit cell angles | 90.00, 120.45, 90.00 |
Refinement procedure
Resolution | ? - 1.900 |
R-factor | 0.184 |
Rwork | 0.184 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | GDHB25 |
RMSD bond length | 0.018 |
RMSD bond angle | 3.800 |
Data reduction software | XENGEN (2.0) |
Data scaling software | XENGEN (V. 2.0) |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.600 |
Rmerge | 0.014 * |
Number of reflections | 1291 |
Completeness [%] | 73.0 |
Redundancy | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | SPERMINE_HCL | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 1 (mM) | single strand concentration |
2 | 1 | drop | sodium cacodylate | 20 (mM) | |
3 | 1 | drop | distamycin hydrochloride | 1 (mM) | |
4 | 1 | drop | magnesium chloride | 20 (mM) | |
5 | 1 | drop | spermine hydrochloride | 1 (mg/ml) | |
6 | 1 | drop | MPD | 3 (%(v/v)) | |
7 | 1 | reservoir | 50 (%) |