300D
CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MN(II)-SOAKED
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Collection date | 1995-09-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.550, 64.550, 135.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.26200 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 16.500 * |
| Data reduction software | DENZO |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.072 |
| Number of reflections | 6716 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 93.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 * | pH 5.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | LI SULFATE | ||
| 3 | 1 | 1 | MN SULFATE | ||
| 4 | 1 | 1 | EDTA | ||
| 5 | 1 | 1 | NA CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 2 | 1 | EDTA | 1.25 (mM) | ||
| 3 | 1 | sodium cacodylate | 50 (mM) |
