2ZXQ
Crystal structure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.96787, 0.97923, 0.97939, 0.96416 |
Spacegroup name | P 65 |
Unit cell lengths | 192.267, 192.267, 123.017 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.490 - 2.000 |
R-factor | 0.17324 |
Rwork | 0.172 |
R-free | 0.19564 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.408 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 172447 | |
<I/σ(I)> | 27.2 | 3.5 |
Completeness [%] | 99.1 | 99.8 |
Redundancy | 7.2 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 0.1M MES-NaOH, 3% PEG 20000, 25% MPD, 0.2M NaCl, 0.01M MnCl2, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |