2ZXQ
Crystal structure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF)
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96787, 0.97923, 0.97939, 0.96416 |
| Spacegroup name | P 65 |
| 格子定数 [Å] | 192.267, 192.267, 123.017 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 36.490 - 2.000 |
| R因子 | 0.17324 |
| Rwork | 0.172 |
| R-free | 0.19564 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.408 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| 独立反射数 | 172447 | |
| <I/σ(I)> | 27.2 | 3.5 |
| 完全性 [%] | 99.1 | 99.8 |
| 冗長性 | 7.2 | 6.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 0.1M MES-NaOH, 3% PEG 20000, 25% MPD, 0.2M NaCl, 0.01M MnCl2, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
