2Y8Q

Structure of the regulatory fragment of mammalian AMPK in complex with one ADP

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-01-12
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
格子定数 [Å]	49.026, 119.918, 130.150
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	20.000 - 2.800
R因子	0.234
Rwork	0.232
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v8q
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.644
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.5)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	2.900
分解能 [Å] (高)	2.800	2.800
Rmerge_l_obs	0.040	0.360
独立反射数	17426
<I/σ(I)>	12.4	2.3
完全性 [%]	93.8	93.5
冗長性	2.7	2.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1