2Y0F
STRUCTURE OF GCPE (IspG) FROM THERMUS THERMOPHILUS HB27
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 84.500, 130.970, 101.260 |
| 格子定数 [度] | 90.00, 102.10, 90.00 |
精密化法
| 残基 | 20.000 - 2.500 |
| R因子 | 0.202 |
| Rwork | 0.199 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.018 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.809 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.6.0093) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.600 |
| 分解能 [Å] (高) | 2.500 | 2.500 |
| Rmerge_l_obs | 0.070 | 0.470 |
| 独立反射数 | 71563 | |
| <I/σ(I)> | 11.8 | 2.2 |
| 完全性 [%] | 96.0 | 86.6 |
| 冗長性 | 3.6 | 2.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 7.5 | PROTEIN SOLUTION 30MM TRIS-HCL PH7.5, 0.15M NACL, 12MG/ML PROTEIN. RESERVOIR/PRECIPITANT 47% MPD, 2% TERT-BUTANOL |
