2XR8
Crystal structure of biphenyl dioxygenase from Burkholderia xenovorans LB400
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-25 |
| Detector | ADSC SX-165 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 132.585, 132.350, 132.984 |
| 格子定数 [度] | 102.60, 102.68, 104.61 |
精密化法
| 残基 | 129.100 - 2.490 |
| R因子 | 0.21649 |
| Rwork | 0.216 |
| R-free | 0.26714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gzy |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.925 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 120.000 | 2.600 |
| 分解能 [Å] (高) | 2.490 | 2.490 |
| Rmerge_l_obs | 0.100 | 0.410 |
| 独立反射数 | 255418 | |
| <I/σ(I)> | 6.8 | 1.4 |
| 完全性 [%] | 89.0 | 30 |
| 冗長性 | 2.8 | 1.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 6 | pH 6 |
