2XO2
Human Annexin V with incorporated Methionine analogue Azidohomoalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-05-03 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 |
Unit cell lengths | 152.708, 152.708, 33.603 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.090 - 2.800 |
R-factor | 0.274 |
Rwork | 0.274 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1anx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.330 |
Number of reflections | 7169 | |
<I/σ(I)> | 5.7 | 2.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 2.0 M AMMONIUM SULFATE, 20 MM CALCIUM CHLORIDE, 0.1 M TRIS/HCL, PH 8.5 |