2XO2

Human Annexin V with incorporated Methionine analogue Azidohomoalanine

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-05-03
Detector	DECTRIS PILATUS 6M
Spacegroup name	H 3
格子定数 [Å]	152.708, 152.708, 33.603
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	19.090 - 2.800
R因子	0.274
Rwork	0.274
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anx
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.3)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	2.950
分解能 [Å] (高)	2.800	2.800
Rmerge_l_obs	0.100	0.330
独立反射数	7169
<I/σ(I)>	5.7	2.3
完全性 [%]	99.7	100
冗長性	2.6	2.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		8.5		2.0 M AMMONIUM SULFATE, 20 MM CALCIUM CHLORIDE, 0.1 M TRIS/HCL, PH 8.5