2XDG
Crystal structure of the extracellular domain of human growth hormone releasing hormone receptor.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-04-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 61 |
Unit cell lengths | 31.648, 31.648, 287.138 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.410 - 1.950 |
R-factor | 0.16742 |
Rwork | 0.165 |
R-free | 0.22288 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x57 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.525 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.710 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.080 | 0.790 |
Number of reflections | 11887 | |
<I/σ(I)> | 14 | 2.6 |
Completeness [%] | 97.6 | 95.9 |
Redundancy | 6.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% PEG6000, 0.15M MGCL2, 10% ETHYLENE GLYCOL, 0.1M TRIS PH7.0 |