2WLG
Crystallographic analysis of the polysialic acid O-acetyltransferase OatWY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-22 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 2 2 2 |
Unit cell lengths | 78.669, 94.393, 100.874 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.900 |
R-factor | 0.19473 |
Rwork | 0.193 |
R-free | 0.23106 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IN-HOUSE STRUCTURE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.342 |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.280 |
Number of reflections | 59306 | |
<I/σ(I)> | 52.8 | 7.2 |
Completeness [%] | 99.0 | 95.2 |
Redundancy | 10 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 100 MM SODIUM ACETATE, 2.25 M AMMONIUM ACETATE, pH 4.6 |