2VSN
Structure and topological arrangement of an O-GlcNAc transferase homolog: insight into molecular control of intracellular glycosylation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-04 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.691, 105.692, 151.036 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.520 - 2.750 |
R-factor | 0.241 |
Rwork | 0.241 |
R-free | 0.26500 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.590 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.100 | 0.500 |
Number of reflections | 36765 | |
<I/σ(I)> | 13.7 | 2.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M LI2SO4, 0.1M TRIS PH 8.5, 22.5% PEG 3350 |