2UZP
Crystal structure of the C2 domain of human protein kinase C gamma.
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 138.503, 138.503, 68.358 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.000 |
| R因子 | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsy |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.313 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 56.080 | 2.110 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.100 | 0.700 |
| 独立反射数 | 45478 | |
| <I/σ(I)> | 15.7 | 3.2 |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 10.2 | 10.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 6.5 | 1.8M AMMONIUM SULPHATE, 15MM COBALT CHLORIDE, 0.1M MES PH6.5, pH 6.50 |
