2QZT
Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 263 |
Detector technology | CCD |
Collection date | 2006-07-13 |
Detector | BRUKER PROTEUM |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.360, 56.440, 50.610 |
Unit cell angles | 90.00, 119.40, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.700 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.23600 |
Structure solution method | MIR |
RMSD bond length | 0.014 |
RMSD bond angle | 1.425 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 24343 | |
<I/σ(I)> | 25.8 | 4.9 |
Completeness [%] | 98.8 | 93.8 |
Redundancy | 3.5 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 2 microl protein @ 10mg/ml was mixed with 2microl mother liquor consisting of 1.6 M sodium citrate, 5% glycerol, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 2 microl protein @ 10mg/ml was mixed with 2microl mother liquor consisting of 1.6 M sodium citrate, 5% glycerol, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 2 microl protein @ 10mg/ml was mixed with 2microl mother liquor consisting of 1.6 M sodium citrate, 5% glycerol, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |