2QX5
Structure of nucleoporin Nic96
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0718 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.121, 82.478, 113.028 |
Unit cell angles | 90.00, 91.11, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
Rwork | 0.233 |
R-free | 0.28500 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.712 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 55097 | |
<I/σ(I)> | 19.9 | 3.7 |
Completeness [%] | 99.5 | 99 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 289 | 18% PEG 3350, 0.1M Bis Tris propane, 0.2M Potassium Thiocyanate, 1mM Cetyltrimethylammonium bromide, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |