2QFO
HSP90 complexed with A143571 and A516383
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-04-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 64.157, 88.755, 98.445 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 20.000 - 1.680 |
| R因子 | 0.246 |
| Rwork | 0.224 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yer |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.114 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNX (2002) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 1.740 |
| 分解能 [Å] (高) | 1.680 | 1.680 |
| 独立反射数 | 61674 | |
| <I/σ(I)> | 12.7 | 5.5 |
| 完全性 [%] | 95.3 | 87.8 |
| 冗長性 | 6.5 | 4.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Protein at 35 mg/ml with a reservoir of 22-30 % (w/v) PEG4000, 0.1 M Tris-HCl PH 8.5, 0.2 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
