2PVO
Crystal srtucture of the ternary complex between thioredoxin f, ferredoxin, and ferredoxin: thioredoxin reductase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97970 |
| Spacegroup name | P 32 2 1 |
| 格子定数 [Å] | 130.426, 130.426, 63.837 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 15.000 - 3.400 |
| Rwork | 0.202 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 3.420 |
| 分解能 [Å] (高) | 3.300 | 7.100 | 3.300 |
| Rmerge_l_obs | 0.195 | 0.069 | 0.675 |
| 独立反射数 | 9413 | ||
| <I/σ(I)> | 4.5 | ||
| 完全性 [%] | 97.0 | 89.1 | 99.5 |
| 冗長性 | 8.2 | 7.2 | 7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
