2OR1
RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION
Experimental procedure
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 148.300, 65.000, 27.700 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | ? - 2.500 |
| R因子 | 0.179 |
| Refinement software | TNT |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 25.000 * |
| 分解能 [Å] (高) | 2.500 * |
| Rmerge_l_obs | 0.123 * |
| 独立反射数 | 6019 * |
| 完全性 [%] | 60.7 * |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 4 * | VAPOR DIFFUSION, temperature 277.00K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 3000 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | DNA | 1 (mM) | ||
| 2 | 1 | protein | 2 (mM) | ||
| 5 | 1 | PEG3000 | 12-14 (%) |
