2OMI
Structure of human insulin cocrystallized with protamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-23 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.520, 61.840, 86.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.100 - 2.240 |
R-factor | 0.20276 |
Rwork | 0.199 |
R-free | 0.26838 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | insulin hexamer R-conformation |
RMSD bond length | 0.022 |
RMSD bond angle | 1.941 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.400 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.107 | 0.286 |
Number of reflections | 15881 | |
<I/σ(I)> | 12.1 | 4.1 |
Completeness [%] | 98.6 | 97.6 |
Redundancy | 6.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 50mM resorcinol, 400mM NaCl, 1.0mg/ml protamine 30mM phosphate buffer, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |